SBIR-STTR Award

Microbial control of postharvest decay in tropical fruits
Award last edited on: 5/21/02

Sponsored Program
SBIR
Awarding Agency
USDA
Total Award Amount
$225,000
Award Phase
2
Solicitation Topic Code
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Principal Investigator
James P Stack

Company Information

EcoScience Produce Systems Corporation

PO Box 3228
Orlando, FL 32802
   N/A
   N/A
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Location: Single
Congr. District: 10
County: Orange

Phase I

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
Phase I year
1994
Phase I Amount
$50,000
The aim of this project is to identify microorganisms capable of preventing postharvest decay in tropical fruits caused by plant pathogenic fungi. Ecoscience has demonstrated success in the microbial control of postharvest fungal decays in pome fruits. We will build on this experience to discover and develop microbial control agents that can prevent infection (e.g., crown rot of banana) or inhibit development of latent infections (e.g., anthracnose of mango) in tropical fruits. Microbes that would have wound colonization competency and ability to prevent fungal-induced decay will be selected. Initial screening will assess, i) ability to colonize the wound site and maintain a population suppressive to the pathogenic fungi, ii) population dynamics in wounds as a function of temperature and pathogen propagules, iii) carbon utilization profiles consistent with wound exudates of the target fruit (phase ii). Screening for ability to prevent decay will involve direct challenge assays. The ultimate objective is to develop biological replacements for the synthetic fungicides currently in use. This approach has the potential to generate a base technology with multiple spin-off products for tropical fruits as well as vegetables.

Phase II

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
Phase II year
1996
Phase II Amount
$175,000
The aim of this project was to identify microorganisms capable of preventing postharvest decay in tropical fruits caused by plant pathogenic fungi. The main focus was crown rot disease of banana caused by fusarium spp. And colleototrichum musae; some effort was also directed at anthracnose of banana caused by c. Musae. Ecoscience has demonstrated success in the development and commercialization of microbial control products for postharvest fungal decays in pome and citrus fruits. Our objective was to discover and develop microbial control agents that can prevent infection (e.G., Crown rot of banana) or inhibit destructive development from latent infections in tropical fruits (e.G., Anthracnose of banana and mango). Microbes with the ability to prevent decay on banana were selected from a variety of sources including pome, citrus and tropical fruits, as well as, three different soil types. Initial screening assessed 1) the ability to grow rapidly under standard culture conditions on a simple medium, and 2) the ability to colonize wound sites and suppress pathogenic fungi. Several isolates were identified with the desired activity. Some isolates prevented only crown rot of banana, some isolates prevented only anthracnose development, and a few showed ability to prevent both diseases.Applications:Postharvest decay of tropical fruits can result in estimated losses of 15% annually. This includes crown rot of banana and anthracnose diseases of many fruits. Fungicides are routinely used to prevent or minimize these losses. Concern with fungicide residues in fruits, the development of reduced sensitivities to fungicides in pathogen populations, and the prevalence of fungicides as environmental contaminants justifies the search for alternatives. The objective is to develop biological supplements and/or replacements for the synthetic fungicides currently in use. This approach has the potential to generate a base technology with multiple spin-off products for tropical fruits and vegetables.