In the Phase I research, BioMetallics Inc., has successfully developed a simple solid phase enzyme immunoassay (EIA) for progesterone, to be used for early pregnancy diagnosis in farm animals. The EIA relies on visual detection and eliminates the need for extraction, centrifugation, or other instrumentation. The assay is highly specific for progesterone and has a sensitivity 0.1-30 pmol (0.03-0.94 ng) progesterone suitable for measurement of progesterone in milk, serum, or plasma samples. In this continuation research, the assay will be adapted to direct EIA for milk progesterone. Covalent attachment of the antibody to a solid phase will be studied in order to insure a uniform amount of antibody. Other heterologous enzyme conjugate labels and color substrates will be investigated in order to enhance the sensitivity and reliability of the assay. Field testing of the EIA assay and production of a monoclonal antibody for progesterone will be conducted in collaboration with researchers at the Veterinary School of the University of North Carolina.
