We will deploy an automated multiplexed live-cell assay to detect and quantify agonistand inverse-agonist activities against 55 GPCRs known to be upregulated ordownregulated in human gliomas. The assay will be used to look for the secretion ofeffectors for any of the 55 receptors by 48 established glioma cell lines of the HumanGlioblastoma Cell Culture Resource. This endeavor may yield new leads towards thedevelopment of anti-tumor therapeutics, and will provide a platform for detecting suchleads in resected tumor tissue and in chemical or biologic libraries in future experiments. In this project we will build and validate an automated multiplexed live-cell array to detect and quantify agonist and inverse-agonist activities against 55 GPCRs known to be upregulated or downregulated in human gliomas. The assay will be used to look for the secretion of effectors for any of the 55 receptors by the 48 established glioma cell lines of the Human Glioblastoma Cell Culture Resource. This endeavor may yield new leads towards the development of anti-tumor therapeutics, and will provide a platform for detecting such leads in resected tumor tissue and in chemical or biologic libraries. Biological Assay ; Assay ; Bioassay ; Biologic Assays ; Malignant Neoplasms ; Cancers ; Malignant Tumor ; malignancy ; neoplasm/cancer ; Cell Culture Techniques ; cell culture ; Cells ; Cell Body ; DNA ; Deoxyribonucleic Acid ; Future ; Glioblastoma ; Grade IV Astrocytic Neoplasm ; Grade IV Astrocytic Tumor ; Grade IV Astrocytoma ; glioblastoma multiforme ; spongioblastoma multiforme ; Glioma ; Glial Cell Tumors ; Glial Neoplasm ; Glial Tumor ; Neuroglial Neoplasm ; Neuroglial Tumor ; glial-derived tumor ; neuroglia neoplasm ; neuroglia tumor ; Human ; Modern Man ; instrumentation ; Libraries ; Ligands ; Resources ; Research Resources ; Computer software ; Software ; Testing ; Transfection ; Conditioned Culture Media ; Conditioned Medium ; image processing ; Biological ; Chemicals ; Individual ; Agonist ; Therapeutic ; Spottings ; System ; Tumor Tissue ; Receptor Protein ; receptor ; receptor downregulation ; expression vector ; novel ; Human Cell Line ; G Protein-Complex Receptor ; G Protein-Coupled Receptor Genes ; GPCR ; G-Protein-Coupled Receptors ; response ; drug discovery ; Cellular Secretion ; Cell secretion ; Cellular Assay ; cell assay ; Resected ; Development ; developmental ; Image ; imaging ; vector ; tumor ; glioma cell line ; experimental study ; experiment ; experimental research ;
