This project, titled Ophthalmic Time-resolved Confocal Scanning Microfluorometer (OTR-CSMF), is aimed at building a dedicated confocal microfluorometer capable of depth-resolved fluorescence spectroscopy across the cornea for reconstruction of a map of physiological parameters (ph, O2, etc.). At the end of Phase I stage, the instrument will be able to perform both intensity and fluorescence lifetime measurements across the cornea at different depths with a high depth resolution (
Public Health Relevance Statement: Project Narrative The transparency of the cornea is essential for perfect vision. We are proposing a novel instrument to assess corneal pathophysiology during the drug development process. The instrument will permit unprecedented depth- resolved measurements of biochemical/biophysical parameters to characterize the health of the cornea.
Project Terms: Affect; analog; Animals; Anterior; Anterior eyeball segment structure; Aqueous Humor; base; Basic Science; Binding; Biochemical; Biological Assay; biophysical properties; Cattle; Characteristics; Communities; Computer software; Confocal Microscopy; Cornea; Corneal Diseases; data acquisition; Data Analyses; design; Development; Devices; Diffusion; digital; drug development; drug discovery; Epithelial; Epithelium; experimental study; extracellular; Eye; Family suidae; Feasibility Studies; Fluorescein; Fluorescence; Fluorescence Microscopy; Fluorescence Spectroscopy; Fluorometry; fluorophore; Frequencies; Fuchs' Endothelial Dystrophy; Functional disorder; fundamental research; Goals; Health; Hour; Human; in vivo; in vivo evaluation; Indiana; innovation; instrument; Investigation; Keratoconus; Kinetics; Label; Legal patent; Maps; Measurement; Measures; Microfluidics; millisecond; Modification; Molecular; nanoparticle; novel; operation; Ophthalmology; Oryctolagus cuniculus; Outcome; Oxygen; Partial Pressure; Permeability; Pharmaceutical Preparations; Pharmacologic Substance; Phase; phase 1 study; Phenanthrolines; Photons; Physiological; Physiology; Pilot Projects; pre-clinical research; Process; Property; prototype; reconstruction; Research; Resolution; Retina; Ruthenium; Scanning; Speed; System; Technology; Testing; Time; time use; Tissues; translational impact; Universities; Validation; Vision
