Memantine, an aminoadamantane, is approved to treat moderate-to-severe Alzheimer's disease in the US and Europe. Memantine selectively inhibits abnormally active N-methyl-D-aspartate-type glutamate receptor (NMDAR) channels, while preserving normal glutamate activity and physiological neuronal function (Lipton, 2006; Lipton, 2007a,b). Pathological NMDA receptor activity is further down-regulated by S-nitrosylation of cysteine residues located on the N-terminus or extracellular domain. Taking advantage of these insights, we have developed a series of bifunctional antagonists, nitromemantines, that not only preferentially bind to the open-channel state but also selectively target NO to a second modulatory site using the memantine pharmacophore as a homing motif. Our data suggest that some of these memantine analogs have good potency, while maintaining selectivity for persistently open NMDAR channels. Most importantly, they appear to have greater neuroprotective properties than memantine in both in vitro and in vivo animal models. Our results provide structural guidance to further optimize, and then identify, a potential development candidate with an optimal profile to maximize neuroprotective effects. PRI owns, and the PI is a co-inventor on the original nitromemantine patents (Wang, 2002, 2003, 2008). Recent work from the laboratory of our collaborator (and co-inventor), Prof. Stuart Lipton, demonstrates the unique superiority of one of our nitromemantines (YQW- 036, 1-amino-3,5-diethyl-7-nitrateadamantane) versus memantine to rescue/protect synapses (Talantova, 2013). This compound preferentially and uniquely modulates pathogenic extrasynaptic NMDARs versus synaptic NMDARs. Phase I seeks to identify a development candidate based on our promising dual-targeted lead compound. Phase II will support translational, IND-enabling studies. The achievement of this milestone will generate a proprietary first-in-class, disease-modifying drug for Alzheimer's disease.
Public Health Relevance Statement: Public Health Relevance: Alzheimer's disease (AD) is a devastating form of dementia whose incidence is increasing due to an aging population. The FDA-approved drug memantine is arguably the best available therapeutic for AD. We have synthesized improved memantine-like drugs and have demonstrated their effectiveness in cell culture and in an animal model. Development of these memantine analogs will result in a best-in-class, disease-modifying treatment for AD.
Project Terms: Achievement; aging population; Alzheimer's Disease; Amyloid beta-Protein; analog; Animal Model; Animals; base; Binding; Biological; Biological Assay; Cell Culture Techniques; Clinical; Cysteine; Data; Dementia; design; Development; Disease; Disease model; Drug Kinetics; effective therapy; Effectiveness; Electrophysiology (science); Epilepsy; Europe; excitotoxicity; Extracellular Domain; FDA approved; Fluorescence; Glutamate Receptor; Glutamates; Goals; Homing; improved; In Vitro; in vivo; Incidence; insight; Laboratories; Lead; Legal patent; Measures; Memantine; N-Methyl-D-Aspartate Receptors; N-Methylaspartate; nervous system disorder; Neurodegenerative Disorders; Neuronal Injury; Neurons; neuroprotection; Nitrates; Nitric Oxide; Nitroglycerin; novel; patch clamp; Peripheral Nervous System Diseases; Pharmaceutical Preparations; Pharmacology; pharmacophore; Phase; Physiological; Plasma; Property; public health relevance; receptor; receptor function; research study; Safety; Series; Site; stroke; Structure; Synapses; Techniques; Therapeutic; Time; Toxicology; Trauma; Treatment Efficacy; Work
