The overarching goal of Phase I is to produce a method that will generate, identify and clone a catalog of protein-protein interactions in a single rapid experiment. In this proposal we will model the technology using antibody-antigen interactions. Recombinant antibody libraries against a proteome could be produced prior to any need, and then desired affinity reagents systematically cloned as needed. Libraries of recombinational biopanning integration product against a proteome could also be distributed along with primer sets against a set of barcodes for facile cloning. In future endeavors, the method will be used to clone tumor-cell or other diseased tissue cDNAs to use as an epitope library to quickly identify potential biomarkers. Successful completion of this phase will allow th commercialization of antibodies that can be used for diagnostic and therapeutic applications at a speed and comprehensiveness that is not easily achieved by current methods. Kits composed of vectors, reagents, strains and methods would be of commercial interest.
Thesaurus Terms: Affinity;Antibodies;Antigen Targeting;Antigens;Base;Bioinformatics;Biological Markers;Biological Response Modifier Therapy;Cataloging;Catalogs;Cell Surface;Cloning;Commercialization;Complementary Dna;Design;Diagnostic;Emulsions;Epitopes;Future;Generations;Goals;Human;Instrument;Integrase;Interest;Libraries;Methods;Modeling;Neoplastic Cell;Novel;Nucleic Acids;Organ;Organism;Peptides;Phase;Pilum;Protein Protein Interaction;Proteins;Proteome;Protocols Documentation;Public Health Relevance;Reagent;Reagent Testing;Recombinant Antibody;Research Study;Screening Result;Specific Qualifier Value;Speed (Motion);Study Models;System;Technology;Testing;Therapeutic;Tissues;Transfer Rna;Tumor;Vector;Viral;
