SBIR-STTR Award

Label-Free Platform for Applications in Drug Discovery
Award last edited on: 1/8/19

Sponsored Program
SBIR
Awarding Agency
NIH : NCATS
Total Award Amount
$1,397,557
Award Phase
2
Solicitation Topic Code
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Principal Investigator
David Wesley Goad

Company Information

SensiQ Technologies Inc

800 Research Parkway Suite 100
Oklahoma City, OK 73104
   (405) 239-8600
   matthew.cumming@sensiqtech.com
   www.sensiqtech.com
Location: Single
Congr. District: 05
County: Oklahoma

Phase I

Contract Number: 1R44TR000618-01
Start Date: 7/1/13    Completed: 12/31/13
Phase I year
2013
Phase I Amount
$320,572
Incremental improvements in label free screening technologies over the past decade have not produced the capability necessary to support the changing needs of drug discovery programs. Persistent artifacts related to non-specific binding, promiscuous binding, precipitation, complex binding, tubing retention, aggregation, and sub-optimally designed platforms continue to hinder progress. Substantial progress has been made in establishing methodologies that maximize the value of the data that is currently available. The proposed platform encompasses a variety of novel adaptations including a dual mode detector exploiting surface plasmon resonance (SPR), which is sensitive to changes at the surface, and bulk solution-sensitive critical angle refractometry (CAR). Taylor dispersion-based continuous titrations (OneStepTM) and stepped analyte titrations (FastStepTM) are combined with an innovative thermal gradient capability to provide a high throughput thermodynamic screening format (DH, DS, DCp) capable of producing over 2000 isotherms/day. The realizable mass sensitivity will approach 30 fg/mm2 while the effects of mismatched buffer conditions are automatically compensated by the CAR response. Dual component titrations allow rapid compound pre-screening covering a large matrix of buffer conditions in a single run. We expect that access to these convenient optimization tools will significantly reduce artifacts and rescue many compounds that would otherwise be discarded. OneStepTM titrations will extend over 3-4 orders in concentration and provide high resolution of multisite binding events. Compound binding usually follows a rapid approach to steady state, allowing the binding curves from each titration to be treated as affinity isotherms. These time- dependent isotherms can be directly fitted with a multi-site affinity model containing dispersion terms in addition to kinetic and/or affinity binding terms for each interaction site that will return estimates of the analyte diffusio coefficient (D) along with the interaction constants (kon, koff, KD).

Public Health Relevance Statement:


Public Health Relevance:
A next generation label-free drug discovery platform tailored for applications from early stage to later stage drug discovery will be developed. The proposed platform combines multiple enhancements in optics, microfluidics, sampling, thermal management, and data analysis that together provide effective solutions to longstanding difficulties encountered when screening compound libraries for new drug candidates or drug fragments. These added capabilities help optimize the test conditions and, more importantly, will provide higher resolution data that support more confident decision in the drug development process. Retaining a high sampling capacity is important because this allows the platform to contribute during the early compound screening phase, before considerable resources are expended, and helps prevent wasted effort on false leads. Phase I will produce a label free optical analyzer module capable of extending the sensitivity of the label-free response by 5-10 fold. More significantly the range of compound interaction speeds/strengths that can be measured will increase by as much as 100-fold. Phase II will integrate this analyzer module into an automated fluidic handling platform complete with pumps, actuators, thermal control systems, and intuitive control/operator software to create an alpha level unit that will be commercialized within 2.5 years.

Project Terms:
Affinity; base; Behavior; Binding (Molecular Function); Biosensor; Buffers; Calorimetry; Complex; Computer software; Data; Data Analyses; Data Quality; design; Detection; detector; drug candidate; drug development; drug discovery; Event; experience; Goals; Heating; high throughput screening; innovation; Kinetics; Label; Lead; Libraries; Measures; meetings; Membrane; Methodology; Methods; Metric; Microfluidics; Modality; Modeling; Morphologic artifacts; next generation; novel; Optics; Pharmaceutical Preparations; Phase; Precipitation; prevent; Process; programs; prototype; public health relevance; Pump; Qualitative Methods; receptor; Refractometry; Refractory; Resolution; Resources; response; Running; Sampling; screening; sensor; Site; Solutions; Speed (motion); Staging; success; Surface; Surface Plasmon Resonance; System; Techniques; Technology; Testing; Thermodynamics; Time; Titrations; tool; Validation; wasting

Phase II

Contract Number: 4R44TR000618-02
Start Date: 00/00/00    Completed: 00/00/00
Phase II year
2014
Phase II Amount
$1,076,985
DESCRIPTION: Incremental improvements in label free screening technologies over the past decade have not produced the capability necessary to support the changing needs of drug discovery programs. Persistent artifacts related to non-specific binding, promiscuous binding, precipitation, complex binding, tubing retention, aggregation, and sub-optimally designed platforms continue to hinder progress. Substantial progress has been made in establishing methodologies that maximize the value of the data that is currently available. The proposed platform encompasses a variety of novel adaptations including a dual mode detector exploiting surface plasmon resonance (SPR), which is sensitive to changes at the surface, and bulk solution-sensitive critical angle refractometry (CAR). Taylor dispersion-based continuous titrations (OneStepTM) and stepped analyte titrations (FastStepTM) are combined with an innovative thermal gradient capability to provide a high throughput thermodynamic screening format (DH, DS, DCp) capable of producing over 2000 isotherms/day. The realizable mass sensitivity will approach 30 fg/mm2 while the effects of mismatched buffer conditions are automatically compensated by the CAR response. Dual component titrations allow rapid compound pre-screening covering a large matrix of buffer conditions in a single run. We expect that access to these convenient optimization tools will significantly reduce artifacts and rescue many compounds that would otherwise be discarded. OneStepTM titrations will extend over 3-4 orders in concentration and provide high resolution of multisite binding events. Compound binding usually follows a rapid approach to steady state, allowing the binding curves from each titration to be treated as affinity isotherms. These time- dependent isotherms can be directly fitted with a multi-site affinity model containing dispersion terms in addition to kinetic and/or affinity binding terms for each interaction site that will return estimates of the analyte diffusio coefficient (D) along with the interaction constants (kon, koff, KD).

Thesaurus Terms:
Affinity;Base;Behavior;Binding (Molecular Function);Biophysical Properties;Biosensor;Buffers;Calorimetry;Complex;Computer Software;Data;Data Analyses;Data Quality;Design;Detection;Detector;Drug Candidate;Drug Development;Drug Discovery;Event;Experience;Goals;Heating;High Throughput Screening;Innovation;Kinetics;Label;Lead;Libraries;Measures;Meetings;Membrane;Methodology;Methods;Metric;Microfluidics;Modality;Modeling;Morphologic Artifacts;Next Generation;Novel;Optics;Pharmaceutical Preparations;Phase;Precipitation;Prevent;Process;Programs;Prototype;Public Health Relevance;Pump;Qualitative Methods;Receptor;Refractometry;Refractory;Resolution;Resources;Response;Running;Sampling;Screening;Sensor;Site;Solutions;Speed (Motion);Staging;Success;Surface;Surface Plasmon Resonance;System;Techniques;Technology;Testing;Thermodynamics;Time;Titrations;Tool;Validation;Wasting;