Membrane proteins are an essential component of intercellular signaling pathways. The overwhelming majority of small molecule drugs, either directly or indirectly, target membrane proteins. Arrays of membrane proteins would be important tools in drug screening and academic research laboratories in studies of interactions between proteins and lipids, proteins and membrane proteins, as well as therapeutic drugs. Although other microarrays technologies such as DNA and antibody microarrays have been well- developed, membrane protein microarray has been under-developed to fulfill the needs in biomedical research and drug screening. Life BioScience Inc. (LBSI) proposes to develop a commercially viable membrane protein array system capable of studying membrane protein function and interactions in a high multiplexed and high throughput manner by overcoming current technical barriers. Phase I studies will focus on developing microarray platform and validating the general applicability of the platform to produce functional membrane protein arrays. In Phase II we will expand the system to higher multiplex G-protein-coupled receptors (GPCRs) microarrays and incorporate a high sensitivity detection system to comprise a complete membrane protein assay platform for protein functional analysis and drug screening applications. This platform will drastically accelerate investigations of protein-lipid, protein-membrane protein, drug-membrane protein, and drug-membrane interactions, and will be useful in the development and screening of drugs targeting a variety of disease states, including cancer, inflammatory diseases, infectious diseases, heart disease, and kidney disease.
Public Health Relevance: Biological membrane proteins play an important role in all living systems. The proposed research in developing membrane protein arrays addresses important needs in the areas of membrane protein function and drug screening and characterization. The membrane protein arrays will be useful in the development of effective and safe therapeutic agents for the treatment of wide range diseases such as cancer, diabetes, inflammation, and cardiac dysfunction.
Thesaurus Terms: Address;Antibodies;Area;Assay;Bioassay;Biochemical;Biologic Assays;Biological;Biological Assay;Biomedical Research;Cancers;Cardiac;Cardiac Diseases;Cardiac Disorders;Cell Function;Cell Membrane Lipids;Cell Process;Cell Membrane;Cell Physiology;Cells;Cellular Function;Cellular Physiology;Cellular Process;Communicable Diseases;Cytoplasmic Membrane;Dna;Dehydration;Deoxyribonucleic Acid;Detection;Development;Development And Research;Diabetes Mellitus;Disease;Disorder;Drug Delivery;Drug Delivery Systems;Drug Screening;Drug Targeting;Drugs;Dysfunction;Environment;Evaluation Studies, Drug, Pre-Clinical;Evaluation Studies, Drug, Preclinical;Fret;Feasibility Studies;Film;Fluorescence Resonance Energy Transfer;Functional Disorder;Future;G Protein-Complex Receptor;G-Protein-Coupled Receptors;Glass;Heart Diseases;Immobilization;Infectious Disease Pathway;Infectious Diseases;Infectious Diseases And Manifestations;Infectious Disorder;Inflammation;Inflammatory;Investigation;Kidney Diseases;Loinc Axis 4 System;Laboratory Research;Laboratory Study;Lead;Life;Ligand Binding;Lipid Bilayers;Lipids;Malignant Neoplasms;Malignant Tumor;Marketing;Measures;Medication;Membrane;Membrane Lipids;Membrane Proteins;Membrane-Associated Proteins;Methods;Microarray Analysis;Microarray-Based Analysis;Modeling;Nephropathy;Organism;Pattern;Pb Element;Peptides;Pharmaceutic Preparations;Pharmaceutical Preparations;Phase;Phase I Study;Physiopathology;Plasma Membrane;Play;Preclinical Drug Evaluation;Printing;Protein Array;Protein Binding;Protein Biochips;Protein Chips;Protein Microarray;Protein Microchips;Proteins;R &D;R&D;Renal Disease;Reporting;Research;Robotics;Role;Screening Procedure;Signal Pathway;Spottings;Subcellular Process;Surface Proteins;System;Therapeutic;Therapeutic Agents;Variant;Variation;Vesicle;Base;Body Water Dehydration;Developmental;Diabetes;Disease/Disorder;Drug Development;Drug Discovery;Drug/Agent;Gene Product;Heart Disorder;Heavy Metal Pb;Heavy Metal Lead;Interest;Kidney Disorder;Lipid Bilayer Membrane;Living System;Malignancy;Membrane Structure;Microarray Technology;Neoplasm/Cancer;Orthopedic Freezing;Pathophysiology;Phase 1 Study;Plasmalemma;Protein Function;Renal Disorder;Research And Development;Screening;Screenings;Small Molecule;Social Role;Success;Therapeutic Target;Tool
