Using a method of retroviral gene transduction we have been able to generate conditionally immortalized long-term hematopoietic stem cell lines from primary human cord blood stem cells. Phase I of this project includes applying newly discovered non- viral technology to generate human cell lines and the subsequent differentiation of those cells into human red blood cells. In addition, the project includes testing the function of these cells in small animals. Phase II of the project will entail optimizing large-scale production of protein transduced long-term hematopoietic stem cells and large-scale production of red blood cells for eventual testing in larger animals and human clinical trials. , ,
Public Health Relevance: This project seeks to optimize a novel method to generate large amounts of human red blood cells for clinical use from human adult stem cells. Once this method has been validated for large-scale production, the long-term goal for benefiting public health will be to use this system to generate red blood cells for clinical applications across a variety of global health conditions including, but not limited to, blood disorders, surgeries and in cases of acute trauma.
Thesaurus Terms: 21+ Years Old;Acute;Adult;Anemia;Animals;Binding;Binding (Molecular Function);Biological Preservation;Blood;Blood Diseases;Blood Precursor Cell;Blood Serum;Blood Erythrocyte;Blood Normocyte;Blood, Cord;Bone Marrow;Cd71;Cell Function;Cell Line;Cell Lines, Strains;Cell Process;Cell Size;Cell Physiology;Cell Surface;Cellline;Cells;Cellular Function;Cellular Physiology;Cellular Process;Chimera Protein;Chimeric Proteins;Clinical;Clinical Trials;Clinical Trials, Unspecified;Data;Dependency;Dependency (Psychology);Drops;Es Cell;Erythrocyte Transfusion;Erythrocytes;Erythrocytic;Flr;Failure (Biologic Function);Fusion Protein;Generations;Genetic;Genome;Goals;Hematologic Diseases;Hematological Disease;Hematological Disorder;Hematopoietic;Hematopoietic Stem Cells;Hemoglobin;Human;Human Cell Line;Human, Adult;Human, General;Immunocompromised;Immunocompromised Host;Immunocompromised Patient;Immunosuppressed Host;In Vitro;Kinetic;Kinetics;Life;Lymphoid Cell;Mammals, Mice;Man (Taxonomy);Man, Modern;Marrow Erythrocyte;Medical;Methods;Mice;Modification;Molecular Interaction;Mother Cells;Murine;Mus;Nature;Nuclear;O Element;O2 Element;Operation;Operative Procedures;Operative Surgical Procedures;Oxygen;Patients;Phase;Preservation, Biologic;Preservation, Biological;Procedures;Progenitor Cells;Progenitor Cells, Hematopoietic;Proteins;Protocol;Protocols Documentation;Public Health;Red Blood Cell Transfusion;Red Blood Cells;Red Cell;Red Blood Corpuscule;Red Cell Of Marrow;Reticuloendothelial System, Blood;Reticuloendothelial System, Bone Marrow;Reticuloendothelial System, Erythrocytes;Serum;Source;Stem Cells;Subcellular Process;Surgical;Surgical Interventions;Surgical Procedure;System;System, Loinc Axis 4;Tfr;Tfrc;Tfrc Gene;Trfr;Trnsf;Technology;Tensile Strength;Testing;Time;Transduction Gene;Transfusion;Trauma;Umbilical Cord Blood;Vector Mediated Transfer Genes;Viral;Adult Human (21+);Aging Population;Base;Blood Corpuscles;Blood Disorder;Cell Type;Clinical Applicability;Clinical Application;Clinical Investigation;Cultured Cell Line;Cytokine;Embryonic Stem Cell;Emotional Dependency;Failure;Fetal;Fetal Cord Blood;Gene Product;Global Health;Human Adult Stem Cell;Immunosuppressed Patient;Irradiation;Large Scale Production;Model Development;New Approaches;New Technology;Novel;Novel Approaches;Novel Strategies;Novel Strategy;Peripheral Blood;Preservation;Progenitor;Public Health Medicine (Field);Public Health Relevance;Reconstitute;Reconstitution;Stem Cell Of Embryonic Origin;Stemness;Surgery
