Approximately 135,000 new cases of non-small cell lung cancer (NSCLC) are diagnosed each year in the USA, yet only 20%-25% of those patients will be diagnosed at a sufficiently early stage to be cured by surgical or other procedures. When used in concert with radiographic imaging, a sensitive and specific blood test for NSCLC could be a clinically valuable and cost-effective tool for early diagnosis. The immune system responds to tumor-associated proteins by secreting autoantibodies long before metastasis occurs. Autoantibody profiling for this important group of circulating tumor markers in peripheral blood has lead us to the identification of appropriate target peptides. Using a T7 phage display library derived from the cDNA of a NSCLC line, we have developed a panel of eight peptides which were formatted as a protein microarray for use in a simple capture immunoassay (Zhong et al. 2006, J. Thoracic Oncol. 1(6), 513). When probed with a blood sample, the array can identify the presence of tumor-associated antibodies in NSCLC but not in normals with both sensitivity and a specificity exceeding 90% at early and later stages of the disease. As used to measure tumor-associated antibodies in serum from a cohort of cancer patients and risk- matched controls, this test affords predictive accuracy that exceeds that of currently available circulating NSCLC- associated protein markers. The overall purpose of this application is to achieve three aims: 1) subclone the DNA sequences encoding the eight peptides identified in our initial discovery effort into two fusion-expression vectors, 2) prepare microarrays of the fusion peptides described in aim 1 and demonstrate that they enable measurement of autoantibody profiles that can distinguish cancer from normal blood samples with unprecedented sensitivity and specificity. 3) Finally, we will acquire a cohort of 30 NSCLC and 30 at risk controls and validate the above peptides as bona fide lung cancer biomarkers using protein microarrays. Microarrays of the purified peptides will be used for rigorous optimization and standardization of all technical and clinical performance measures for the multi-target immunoassay. Our initial clinical application will be early detection of lung cancer, although multiple applications in lung cancer management are rational. Our data shows feasibility and proof of concept that supports the rationale for further development and testing of this approach. A subsequent Phase II application will lead to use of an appropriate platform for definitive marker validation trials for application to clinical screening of NSCLC. Thus the primary goal of this application is to develop a novel blood test for NSCLC that can be rapidly translated into clinical practice.
Public Health Relevance: A blood test for early detection of lung cancer could provide a cost-effective screening approach, and in concert with CT scanning would enable a viable strategy for reducing the severe mortality rate of this disease.
Public Health Relevance Statement: Project narrative: Relevance to Public Health: A blood test for early detection of lung cancer could provide a cost-effective screening approach, and in concert with CT scanning would enable a viable strategy for reducing the severe mortality rate of this disease.
Project Terms: Address; Advanced Development; Agreement; Antibodies; Applications Grants; Area; Arm; Assay; Autoantibodies; Bacteriophage T7; Bacteriophages; Benign; Bioassay; Biologic Assays; Biological Assay; Biosynthetic Proteins; Blood; Blood Plasma; Blood Sample; Blood Serum; Blood Tests; Blood specimen; Businesses; CAT Scan, X-Ray; CAT scan; CT X Ray; CT scan; Cancer Control; Cancer Control Science; Cancer Diagnostics; Cancer Patient; Cancer of Lung; Cancers; Carcinoma, Non-Small-Cell Lung; Chest; Chimera Protein; Chimeric Proteins; Clinical; Coliphage T7; Collaborations; Complementary DNA; Computed Tomography; Computerized Axial Tomography (Computerized Tomography); Computerized Tomography, X-Ray; Curative Surgery; DNA Sequence; DNA, Complementary; Data; Development; Development and Research; Diagnosis; Diagnostic; Diagnostic Method; Diagnostic Procedure; Diagnostic Technique; Disease; Disorder; Doctor of Medicine; Doctor of Philosophy; EDRN; EMI scan; Early Detection Research Network; Early Diagnosis; Elements; Enterobacteria phage T7; Equipment and supply inventories; Exposure to; Fusion Protein; Glass; Goals; Grant; Grant Proposals; Grants, Applications; Head; Hematologic Tests; Hematological Tests; Hematology Testing; Human Resources; Image; Immune system; Immunoassay; In Vitro; Intellectual Property; Intervention; Intervention Strategies; Inventory; Investigators; Journals; Kentucky; Lead; Libraries; Licensing; Lung Cancer screening; Lung Neoplasms; Lung diseases; M.D.; Magazine; Malignant Neoplasms; Malignant Tumor; Malignant Tumor of the Lung; Malignant neoplasm of lung; Manpower; Marker Discovery; Measurement; Measures; Medical; Metastasis; Metastasize; Metastatic Neoplasm; Metastatic Tumor; Methods; Methods and Techniques; Methods, Other; Mortality; Mortality Vital Statistics; NDUL; NSCLC; NSCLC - Non-Small Cell Lung Cancer; National Detection Research Network; Neoplasm Metastasis; Nodule; Non-Small Cell Lung Cancer; Non-Small-Cell Lung Carcinoma; Operation; Operative Procedures; Operative Surgical Procedures; Patients; Pb element; Peptide Library; Peptides; Performance; Ph.D.; PhD; Phage Display; Phages; Phase; Plasma; Procedures; Protein Biochips; Protein Chips; Protein Microarray; Protein Microchips; Proteins; Public Health; Publications; Pulmonary Cancer; Pulmonary Diseases; Pulmonary Disorder; Pulmonary Neoplasms; Pulmonary malignant Neoplasm; R & D; R&D; Radiation; Reagent; Receiver Operating Characteristics; Recombinant Proteins; Recombinants; Research Personnel; Researchers; Respiratory Disease; Respiratory Disorder; Respiratory System Disease; Respiratory System Disorder; Reticuloendothelial System, Blood; Reticuloendothelial System, Serum, Plasma; Rights; Risk; Running; SBIR; SBIRS (R43/44); Sampling; Scientific Publication; Screening for Lung Cancer; Screening procedure; Secondary Neoplasm; Secondary Tumor; Sensitivity and Specificity; Serum; Serum, Plasma; Slide; Small Business Innovation Research; Small Business Innovation Research Grant; Smoker; Specificity; Spottings; Staging; Standardization; Surgical; Surgical Interventions; Surgical Procedure; Survival Rate; T7 Phage; TM-MKR; Techniques; Testing; Thorace; Thoracic; Thoracic Oncology; Thorax; Time; Tomodensitometry; Tomography, Xray Computed; Translating; Translatings; Tumor Cell Migration; Tumor Markers; Tumor of the Lung; Ubiquitin Like Proteins; Universities; Upper arm; Validation; Work; X-Ray Computed Tomography; autoimmune antibody; bacterial virus; base; biomarker; body system, allergic/immunologic; cDNA; cancer diagnosis; cancer metastasis; catscan; clinical applicability; clinical application; clinical practice; cohort; computed axial tomography; computerized axial tomography; computerized tomography; cost; disease/disorder; early detection; expression vector; gene product; heavy metal Pb; heavy metal lead; high risk; imaging; improved; interest; interventional strategy; language translation; lung cancer; lung cancer early detection; lung disorder; malignancy; maltose-binding protein; neoplasm/cancer; nonsmall cell lung cancer; novel; organ system, allergic/immunologic; patient population; peripheral blood; personnel; prototype; public health medicine (field); public health relevance; ray (radiation); research and development; screening; screenings; self reactive antibody; surgery; synthetic peptide; tool; tumor; validation studies
