The aim of this project is to expand the capabilities of a novel `liquid array' platform, based on Encoded Sortable Particle (ESP) technology to make it cost and performance compatible with high multiplex assays. ESPs are optically-encoded microfabricated particles that can be manipulated using magnetic force. We will adapt our ESP-based liquid array system, which has already been successfully applied in the low multiplex space, to accommodate assays of 100,000-plex or more. ESP technology has broad applicability and should be a valuable tool in the diagnosis, prognosis, and characterization of a variety of disease states, including cancer, heart disease, arthritis and inflammatory diseases, kidney disease, liver disease, allergic responses, and infectious diseases (e.g. HIV).
Public Health Relevance: ESP technology as applied to the high multiplex assay space will provide a broad platform for many different kinds of assays. The ESP platform is intrinsically versatile, and has the potential to be used in basic biomedical research, as well as high- throughput discovery and clinical diagnostics. The ability to control the particles magnetically (in a manner significantly different from magnetic beads) is a significant advantage. This technology should be useful in the diagnosis and prognosis of a variety of disease states, including cancer, heart disease, arthritis and inflammatory diseases, kidney disease, and infectious diseases (e.g. HIV).
Public Health Relevance Statement: Project Narrative ESP technology as applied to the high multiplex assay space will provide a broad platform for many different kinds of assays. The ESP platform is intrinsically versatile, and has the potential to be used in basic biomedical research, as well as high- throughput discovery and clinical diagnostics. The ability to control the particles magnetically (in a manner significantly different from magnetic beads) is a significant advantage. This technology should be useful in the diagnosis and prognosis of a variety of disease states, including cancer, heart disease, arthritis and inflammatory diseases, kidney disease, and infectious diseases (e.g. HIV).
Project Terms: AIDS Virus; Acquired Immune Deficiency Syndrome Virus; Acquired Immunodeficiency Syndrome Virus; Allergic; Area; Assay; Atrophic Arthritis; Bioassay; Biologic Assays; Biological Assay; Biomedical Research; Cancers; Cardiac Diseases; Cardiac Disorders; Clinical; Code; Coding System; Combinatorial Synthesis; Communicable Diseases; DNA Chips; DNA Microarray; DNA Microarray Chip; DNA Microchips; Development; Diagnosis; Diagnostic; Disease; Disorder; Flexibility; Forecast of outcome; HIV; HTLV-III; Heart Diseases; Hepatic Disorder; Human Immunodeficiency Viruses; Human T-Cell Leukemia Virus Type III; Human T-Cell Lymphotropic Virus Type III; Human T-Lymphotropic Virus Type III; In Situ; Individual; Infectious Disease Pathway; Infectious Diseases; Infectious Diseases and Manifestations; Infectious Disorder; Inflammatory Arthritis; Kidney Diseases; LAV-HTLV-III; Libraries; Liquid substance; Liver diseases; Lymphadenopathy-Associated Virus; Magnetism; Malignant Neoplasms; Malignant Tumor; Marketing; Method LOINC Axis 6; Methodology; Methods and Techniques; Methods, Other; Microscope; Nephropathy; Oligo; Oligonucleotides; Optics; Performance; Phase; Pliability; Process; Prognosis; Property; Property, LOINC Axis 2; Protein Biochips; Protein Chips; Protein Microarray; Protein Microchips; Proteins; Reader; Reading; Renal Disease; Rheumatoid Arthritis; SBIR; SBIRS (R43/44); Sampling; Semiconductors; Small Business Innovation Research; Small Business Innovation Research Grant; Surface; System; System, LOINC Axis 4; Techniques; Technology; Virus-HIV; Work; base; cost; design; designing; disease/disorder; experiment; experimental research; experimental study; fluid; gene product; heart disorder; hepatopathy; kidney disorder; large scale production; liquid; liver disorder; magnetic; magnetic beads; malignancy; meetings; neoplasm/cancer; novel; outcome forecast; particle; prevent; preventing; protein expression; prototype; public health relevance; renal disorder; research study; resilience; response; success; tool
