Inhalants, which include aerosols, volatile liquids, gases, and nitrites, have found increasing use as mind-altering drugs especially among middle school- and high school-aged children. Inhalants have devastating effects on the nervous system, as well as the lungs, liver, and circulatory system. This SBIR proposal addresses the need to teach about the effects of inhalants in high school biology using Sf9 insect cell culture, a system that lends itself to high schools in terms of its affordability, reproducibility, and relative ease of use. Insect cell will be incubated in the presence of inhalants and cells toxicity determined by cell growth and survival, as well as measurements of apoptosis vs. necrosis as the form of cell death. We will also develop assessment tools for educational strategies to prevent abuse of inhalants that are linked to the National Science Education Standards.
Public Health Relevance: Most high schools have not utilized insect cell culture for laboratory teaching activities. While cell culture studies are copiously published, little has been done to fulfill the needs for high school biology, technology, or multi-disciplinary sciences. We propose to make these available in a format that also teaches the dangers of misuse of household items as drugs.,
Public Health Relevance Statement:, Layman Abstract Most high schools have not utilized insect cell culture for laboratory teaching activities. While cell culture studies are copiously published, little has been done to fulfill the needs for high school biology, technology, or multi-disciplinary sciences. We propose to make these available in a format that also teaches the dangers of misuse of household items as drugs.,
Project Terms:, 0-11 years old; Address; Aerosols; Apoptosis; Apoptosis Pathway; Assay; Atmosphere; Atmosphere, planetary; Bioassay; Biologic Assays; Biologic Sciences; Biological Assay; Biological Sciences; Biology; CO2; Carbon Dioxide; Carbonic Anhydride; Cardiovascular; Cardiovascular Body System; Cardiovascular system; Cardiovascular system (all sites); Cell Culture Techniques; Cell Death; Cell Death, Programmed; Cell Survival; Cell Viability; Cell division; Cells; Cellular Expansion; Cellular Growth; Chemicals; Child; Child Youth; Children (0-21); Cultured Cells; Drug abuse; Drugs; Educational process of instructing; Exercise; Exercise, Physical; Gases; Generalized Growth; Growth; Hand; Health; Hearing; Household; Human, Child; Humidity; Incubated; Incubators; Individual; Inhalant dose form; Insecta; Insects; Invertebrates, Insects; Laboratories; Learning; Life Sciences; Link; Liquid substance; Liver; Lung; Measurement; Measures; Medication; Mind; Modeling; NRVS-SYS; Necrosis; Necrotic; Nervous System; Nervous system structure; Neurologic Body System; Neurologic Organ System; Nitrites; Organ System, Cardiovascular; Pharmaceutic Preparations; Pharmaceutical Preparations; Phase; Plastics; Publishing; Relative; Relative (related person); Reproducibility; Research; Research Design; Respiratory System, Lung; SBIR; SBIRS (R43/44); School-Age Population; Science; Small Business Innovation Research; Small Business Innovation Research Grant; Solvents; Students; Study Type; System; System, LOINC Axis 4; Teaching; Technology; Teen; Teenagers; Teens; Temperature; Tissue Growth; Toxic effect; Toxicities; Toxicology; United States; Vascular, Heart; abstracting; abuse of drugs; abuses drugs; base; body system, hepatic; cell determination; cell growth; children; circulatory system; design; designing; drug/agent; experience; experiment; experimental research; experimental study; fluid; forgetting; hearing perception; high school; inhalant; inhalant abuse; inhalation drug abuse; liquid; middle school; necrocytosis; ontogeny; organ system, hepatic; planetary Atmosphere; prevent; preventing; public health relevance; pulmonary; research study; school age; science education; sniffing drug; sound perception; study design; teen years; tissue culture; tool; youngster
