The main goal of this project is a development of the high throughput data acquisition system based on single photon spectrometer which enables detection and highly accurate recognition of sets containing millions of stochastically encoded microparticles and the validation of this system using genome-wide copy number analysis as a model system. As an outcome of this project, we expect dramatic advancement in quantitative molecular biology and medicine, because it will enable true absolute measurements of nucleic molecules and genetic markers of gene-related diseases independent of experimental designs, chemistries, assumptions, etc. The ensuing data will be standardized, and will become comparable between different experiments, tests, or clinical trials conducted at various sites. Moreover, national databases will be accumulated as a result of extensive population-wide tests/measurements. Finally, based only on such data it will become possible to establish norm and pathology ranges, cut-off criteria or threshold levels of the nucleic species for molecular diagnosis of gene-based disorders, and, thereby, to actually establish the field of molecular diagnostics.,
Public Health Relevance Statement:, PROJECT NARRATIVE As an outcome of this project, we expect dramatic advancement in quantitative molecular biology and medicine, because it will enable true absolute measurements of nucleic molecules and genetic markers of gene-related diseases independent of experimental designs, chemistries, assumptions, etc. The ensuing data will be standardized, and will become comparable between different experiments, tests, or clinical trials conducted at various sites. Moreover, national databases will be accumulated as a result of extensive population-wide tests/measurements. Finally, based only on such data it will become possible to establish norm and pathology ranges, cut-off criteria or threshold levels of the nucleic species for molecular diagnosis of gene-based disorders, and, thereby, to actually establish the field of molecular diagnostics.,
Project Terms:, Amplifiers; Biological Models; Biophotonics; Blood capillaries; Capillaries; Capillary; Capillary, Unspecified; Chemistry; Code; Coding System; Color; Computer Programs; Computer software; Computers; Conduct Clinical Trials; DNA Molecular Biology; Data; Data Banks; Data Bases; Databank, Electronic; Databanks; Database, Electronic; Databases; Detection; Development; Diagnostic; Disease; Disorder; Engineering; Engineerings; Experimental Designs; Funding; Genes; Genetic Markers; Goals; Japan; Manuals; Measurement; Medicine; Model System; Models, Biologic; Molecular; Molecular Biology; Molecular Diagnosis; NIH; National Institutes of Health; National Institutes of Health (U.S.); New York; Outcome; Pathology; Phase; Photons; Physiologic pulse; Population; Protocol; Protocols documentation; Pulse; Pump; Q-Dot; Quantum Dots; Reader; Schools, Medical; Science of Chemistry; Science of Medicine; Site; Software; Speed; Speed (motion); System; System, LOINC Axis 4; Technology; Testing; United States National Institutes of Health; Universities; Validation; base; capillary; clinical data repository; clinical data warehouse; computer program/software; data acquisition; data repository; detector; disease/disorder; experiment; experimental research; experimental study; genome-wide; medical schools; new technology; particle; relational database; research study; technology development
