Phase II year
2009
(last award dollars: 2010)
Methods for the delivery of therapeutics, tracking dyes, genes and other substances into complex neural tissue, organotypic cultures and cell types are limited in their effectiveness. The biolistic process, based on acceleration of carrier particles to sufficient velocity, has recently been shown to be a relatively effectual delivery method for neuronal systems. Cell damage and tissue death mediated by the current delivery devices however, limit more widespread use of this process, especially for clinical applications. We propose in this Phase II program, in collaboration with our STTR partner, to expand upon our Phase I development of a novel ballistic device that can be used for delivery of materials to micro-targeted areas, as small as 25 5m, in cells and tissues with minimal trauma. We improved the device design during Phase I so that precise doses of carrier particles and molecules are reproducibly delivered to defined spatial regions. As part of Phase II, additional functional improvements to the device such as a fully automated version will enable high-throughput, programmable, targeted delivery to neural tissues and cells. At the end of our Phase II research we will have developed a modular device that allows the end-user to reproducibly deliver a specified dose of a macromolecule of interest to a pre-selected defined target using a single device. Importantly, this device will have been demonstrated to be effective for delivery in a variety of biological model systems. The use of this system will aid neurobiologists in their understanding of brain development and function, be of use to scientists focusing on cell biology, plants, and other related fields, and be a valuable tool for medical researchers for the delivery of diagnostic and therapeutic compounds.
Public Health Relevance: The proposed research will lead to the further development of a novel pneumatic ballistic delivery device that can be used by the biomedical research community to enhance the basic understanding of disease processes. In addition, this product may have a direct impact on public health by enabling delivery of clinically important macromolecules to cells and tissues of interest.
Public Health Relevance Statement: The proposed research will lead to the further development of a novel pneumatic ballistic delivery device that can be used by the biomedical research community to enhance the basic understanding of disease processes. In addition, this product may have a direct impact on public health by enabling delivery of clinically important macromolecules to cells and tissues of interest.
Project Terms: Acceleration; Acid Maltase Deficiency Disease; Address; Adopted; Agriculture; Alzheimer; Alzheimer disease; Alzheimer sclerosis; Alzheimer syndrome; Alzheimer's; Alzheimer's Disease; Alzheimers Dementia; Alzheimers disease; Anti-Sense Oligonucleotides; Antisense Agent; Antisense Oligonucleotides; Area; Aspiration, Mechanical; Au element; Ballistics; Basic Behavioral Science; Biolistic; Biolistics; Biological; Biological Models; Biology; Biomedical Research; Biotechnology; Bladder; Blast Cell; Blasts; Blood capillaries; Body Tissues; Brain; Cancers; Capillaries; Capillary; Capillary, Unspecified; Cell Growth and Maintenance; Cell Maintenance; Cells; Cellular biology; Cellular injury; Cessation of life; Circadian Rhythms; Clinical; Collaborations; Coloring Agents; Communities; Complex; Computers; Coronary Arteriosclerosis; Coronary Artery Disease; Coronary Artery Disorder; Coronary Atherosclerosis; Cultured Cells; Death; Dementia, Alzheimer Type; Dementia, Primary Senile Degenerative; Dementia, Senile; Dendritic Cells; Dermal; Development; Development and Research; Device Designs; Devices; Diagnostic; Disease; Disorder; Diurnal Rhythm; Dose; Drainage, Suction; Drug Delivery; Drug Delivery Systems; Drug Targeting; Drug Targetings; Drugs; Dyes; Economic Income; Economical Income; Educational process of instructing; Effectiveness; Encephalon; Encephalons; Evaluation; Ferrata cell; Foundations; Gases; Gene Delivery; Gene Expression Inhibitor; Gene-Gun Technique; Generalized Glycogenosis; Genes; Glycogen storage disease type II; Glycogenosis 2; Glycogenosis Type II; Goals; Gold; Government; Hand; Head; Hearing; Hematohistioblast; Hemocytoblast; Hemohistioblast; Huntington Chorea; Huntington Disease; Huntington's; Huntington's Disease; Huntington's Disease Pathway; Huntingtons Disease; INFLM; Income; Inflammation; Injection of therapeutic agent; Injections; Investigators; Laboratories; Laboratory Research; Lead; Lysosomal alpha-1,4-Glucosidase Deficiency Disease; Malignant Neoplasms; Malignant Tumor; Marketing; Mediating; Medical; Medication; Melanogenesis; Metabolic; Methods; Methods and Techniques; Methods, Other; Model System; Models, Biologic; Morphology; NIMH; National Institute of Mental Health; National Institute of Mental Health (U.S.); Nerve Cells; Nerve Unit; Nervous; Nervous System, Brain; Neural Cell; Neurocyte; Neurons; Neurosciences; Nyctohemeral Rhythm; Oligonucleotides, Antisense; Pain; Painful; Patients; Pb element; Persons; Pharmaceutic Preparations; Pharmaceutical Agent; Pharmaceutical Preparations; Pharmaceuticals; Pharmacologic Substance; Pharmacological Substance; Phase; Plants; Plants, General; Pompe Disease; Pressure; Pressure- physical agent; Price; Primary Senile Degenerative Dementia; Process; Programs (PT); Programs [Publication Type]; Progressive Chorea, Hereditary, Chronic (Huntington); Protocol; Protocols documentation; Public Health; R & D; R&D; Reproducibility; Research; Research Personnel; Researchers; Retinal; STTR; Sales; Scientist; Signal Pathway; Slice; Small Business Technology Transfer Research; Specific qualifier value; Specified; Staging; Structure; Suction; Surface; System; System, LOINC Axis 4; Teaching; Techniques; Technology; Therapeutic; Tissues; Toxic effect; Toxicities; Transfection; Trauma; Tungsten; Twenty-Four Hour Rhythm; United States National Institute of Mental Health; Universities; Urinary System, Bladder; Vacuum; Veiled Cells; W element; Wolfram; Wound Healing; Wound Repair; acid maltase deficiency; aerobic respiration control protein; agricultural; alpha 1,4 glucosidase deficiency; arcA protein; base; capillary; cell biology; cell damage; cell injury; cell type; circadian; circadian process; clinical applicability; clinical application; commercialization; daily biorhythm; dementia of the Alzheimer type; density; design; designing; disease/disorder; diurnal variation; drug/agent; dye protein; function improvement; functional improvement; gene gun; hearing perception; heavy metal Pb; heavy metal lead; human disease; improved; in vitro Model; in vivo Model; innovate; innovation; innovative; instrument; interest; macromolecule; malignancy; meetings; neoplasm/cancer; neural; neuronal; novel; particle; pressure; pricing; primary degenerative dementia; programs; prototype; public health medicine (field); public health relevance; relating to nervous system; research and development; response; senile dementia of the Alzheimer type; site targeted delivery; soft tissue; sound perception; targeted delivery; tissue repair; tool; urinary bladder
