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SBIR-STTR Award
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SBIR-STTR Award
10
Selecting New Coding Sequences by PCR with CDNA Primers
Award last edited on: 10/11/19
Sponsored Program
STTR
Awarding Agency
NIH : NIGMS
Total Award Amount
$629,426
Award Phase
2
Solicitation Topic Code
-----
Principal Investigator
Pudur Jagadeeswaran
Company Information
Kalgen Inc
7400 Louis Pasteur Suite 105B
San Antonio, TX 78229
(210) 567-3825
klebe@uthscsa.edu
N/A
Research Institution
University of Texas - San Antonio
Phase I
Contract Number:
1R41GM053373-01
Start Date:
00/00/00
Completed:
00/00/00
Phase I year
1995
Phase I Amount
$100,000
The purpose of this Phase I Small Business Technology Transfer project is to test the utility of a PCR chimeric cDNA selection (CDS) method for isolating unknown coding sequences from larger human genomic contigs. The CDS method is based on the principle of recombining two homologous DNAs. Thus the method will be used to generate chimeric human EST-zebrafish cDNA libraries having the human EST sequences at the 5'-ends and homologous zebrafish cDNA at the 3'-ends. These chimeric cDNAs could be used to map human ESTs in zebrafish mutant genomes. Another application of the CDS method will be to generate a human housekeeping cDNA library that should be useful in identifying tissue specific sequences
Phase II
Contract Number:
2R42GM053373-02
Start Date:
00/00/00
Completed:
00/00/00
Phase II year
1998
(last award dollars: 1999)
Phase II Amount
$529,426
The purpose of this Phase II Small Business Technology Transfer project is to test the utility of a PCR chimeric cDNA selection (CDS) method for isolating unknown coding sequences from larger human genomic contigs. The CDS method is based on the principle of recombining two homologous DNAs. Thus the method will be used to generate chimeric human EST-zebrafish cDNA libraries having the human EST sequences at the 5'-ends and homologous zebrafish cDNA at the 3'-ends. These chimeric cDNAs could be used to map human ESTs in zebrafish mutant genomes. Another application of the CDS method will be to generate a human housekeeping cDNA library that should be useful in identifying tissue specific sequences
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