The human parvovirus B19 (HPV B19), the agent of erythema contagiosum, aplastic crisis, and hydrops fetalis among other disease states, cannot be cultivated using conventional methods and is difficult to collect from patients. This project will develop an assay to detect serum antibodies against HPV B19, using synthetic peptides as capture antigens. First, major epitopes were localized on the viral surface by simultaneously synthesizing on polyethylene pins successive overlapping decapeptides spanning the entire protein in a format allowing ELISA testing. In a second step, peptide epitopes from chosen protein areas were designed, synthesized, modified, and tested in prototype assays using panels of characterized antisera.The present project has demonstrated the possibility of designing synthetic antigens that are effective in viral immunoassays. This can be standardized and made applicable to more complex viruses.Awardee's statement of the potential commercial applications of the research:The immediate application is a solid-phase-type ELISA for detecting IgG and IgM antibodies against HPV B19 in human serum.
Thesaurus Terms: Chemical Synthesis, Design And Production, Communicable Diseases Diagnosis, Diagnostic Tests, Design, Development And Evaluation Of Diagnostic Tests, Immunological Tests And Immunoassay, Enzyme-Linked Immunosorbent Assay (Elisa), Immunological Tests And Immunoassay, Immunological Tests, Peptides, Synthetic Peptides, Viruses, Parvoviridae Diagnostic Quality-Standards, Globulins, Gamma Globulins, Immunoglobulin G, Globulins, Gamma Globulins, Immunoglobulin M, Immunological Tests And Immunoassay, Epitope Mapping, Immunology, Antibody Specificity, Immunology, Antigen-Antibody Reactions, Antigen Processing And Presentation, Immunology, Antigens Viral, Microbial Identification And Classification, Viral Typing And Identification, Proteins, Viral Proteins Biomedical Systems Automated, Computer Processing Of Clinical Data, Chemistry, Analytical Methods, Spectrometry, Immunology, Serology National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
