The purpose of this project was to develop a prototype comprehensive microbiological system suitable for use in microgravity. A unique, thin-film system was investigated which provides for the isolation and identification of bacterial, viral, and fungal pathogens and, where appropriate, allows for determination of antibiotic susceptibility. The film is stored in a desiccated state stable at room temperature for extended periods and is rehydrated immediately prior to use. Only small amounts of liquids are required, thus reducing the likelihood of infectious aerosol formation when in use. Film size is 1 mm x 7.5 cm x 2.5 cm.During Phase I testing of this system, 100 percent sensitivity and specificity was obtained in the identification of various species of bacteria. The dry-film format also proved to be effective in determining bacterial susceptibility to antibiotics. Other research showed how this innovation could be applied in the detection of viruses. The prototype system developed in this project is readily usable in an automated reader or in visual analysis.Potential Commercial Application:A need exists for a reduced-space, modular, microbiology work station in both the physician's office and third world hospital facilities.STATUS: Phase I Only
