Automated multiparametric microscopic cell analysis depends heavily on quantitative specimen preparation techniques. These techniques usually require a laboratory setup of some complexity and often require significant technical performance time. With the aid of computer simulations, a fully automated specimen preparation system will be designed consisting of a small, disposable molded plastic device (the "SpeePrep") that contains, in miniaturized form, the laboratory elements required to quantitatively prepare cells for flow cytometry, cell sorting, or imaging microscopy. Plans also include the design of a small, self-contained "Specimen Handler" that will accomplish the processing of the SpecPrep under microcomputer control without operator intervention. The device will consist of an entry port, measuring system, mixing, staining, washing, and pumping systems, and a series of preloaded reagent reservoirs. A variety of entry ports and specimen delivery options will make the SpecPrep extremely flexible. Automation miniaturization requirements for various templates and processes will be explored. Miniaturization requirements for the SpecPrep processing of microbial and nonbiologic specimens also will be explored.Anticipated Results/Potential Commercial Applications as described by the awardee:This project will define the requirements of the SpecPrep design, assess the range of feasible applications, and explore several prototype designs of differing complexity. This specimen preparation microsystem will replace most of the laboratory requirements for the preparation of cells and other samples for flow cytometry/cell sorting and microspectrophotometry. Safely disposable, it will allow total control of internal conditions, combining unusual convenience, unequivocal labeling, internal standards, full replicability, and low cost per unit. The commercial applications for such a device are potentially numerous, including ones in medical research, engineering, and industrial laboratories.
