This project will demonstrate the feasibility of detection and classification of pathogenic microbes by a fully automated system which will operate in a wide range of military, space, and civilian environments. Because of its military relevance, prevalence, morbidity, mortality, and diagnostic and therapeutic complexity, malaria will be studied as a prototypic disease. In phase I, the detailed spectral characteristics of the internal-constituents of unspained and cytochemically stained malaria parasites and infected host erythrocytes will be determined. Fluorescent antibodies directed against antigens of the parasites will be tested for suitability as quantitative immunofluorescent probes. Diagnostic clues - for detection and speciation of malaria parasites found in these pilot studies will be evaluated for incorporation into a reliable, low-cost, disposable device useful in the military medical context ranging from the base hospital to the battle zone. The results will also guide the exploration of automated detection and classification of other pathogens of military importance.
