News Article

Digital counting of individual molecules by stochastic attachment of diverse label-tags
Date: Nov 01, 2012
Source: ( click here to go to the source)

Featured firm in this article: Cellular Research Inc of Menlo Park, CA

Publication number WO2012148477 A1
Publication type Application
Application number PCT/US2011/065291
Publication date Nov 1, 2012
Filing date Dec 15, 2011
Priority date Dec 15, 2010
Also published as US20110160078
Inventors Stephen P.A. FODOR, Glenn K. Fu
Applicant Cellular Research, Inc.
Patent Citations (123), Non-Patent Citations (104), Referenced by (1), Classifications (3), Legal Events (1)

Compositions, methods and kits are disclosed for high-sensitivity counting of individual molecules by stochastic labeling of a identical molecules in mixtures of molecules by attachment of a unique label-tags from a diverse pool of label tags to confer uniqueness to otherwise identical or indistinguishable events. Individual occurrences of target molecules randomly choose from a non- depleting reservoir of diverse label-tags. Labeled molecules may be detected by hybridization or sequencing based methods. Molecules that would otherwise be identical in information content are labeled to create a separately detectable product that can be distinctly detected. The disclosed stochastic transformation methods reduce the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed label-tags are present. The methods may be used, for example, to count a given species of molecule within a sample.