ChromaTan has developed Continuous Countercurrent Tangential Chromatography (CCTC) a new column-free process that provides a scalable, disposable and cost saving alternative to column chromatography. Purification of proteins and vaccines is vital to biotechnology. Innovation in this industry has lagged, leading to manufacturing bottlenecks. This is especially apparent in antibody production and rapid response pandemic manufacturing. Providing continuous and single-use chromatography solutions for the biotechnology industry, one of the firm's biggest projects, funded by the FDA, seeks to improve product quality, enable flexible manufacturing facilities and cut costs for life-saving antibody therapies. ____ CCTC purification can be described as the typical process steps found in column chromatography happening simultaneously, with the notable exception of sanitization due to its closed-loop nature. In the pipe diagram above an initial capture step is modeled. Each step represents a new buffer condition and the stage inside those steps is comparable to an individual column volume of buffer loaded on to a column. The washout of buffer from one step to another is dictated by the number of stages in the step and gamma the ratio of flow between the retentate and permeate of the step. To ensure complete washout of each step, 3 5 stages are used in each non-binding step.
