Novel Treatment for Dic
Award last edited on: 8/5/19

Sponsored Program
Awarding Agency
Total Award Amount
Award Phase
Solicitation Topic Code

Principal Investigator
Miguel Angel Cruz

Company Information

A2 Therapeutics Inc

One Baylor Plaza
Houston, TX 77030
   (713) 795-0105

Research Institution

Baylor College of Medicine

Phase I

Contract Number: 1R41HL135942-01A1
Start Date: 8/20/18    Completed: 7/31/19
Phase I year
Phase I Amount
Several diseases, including endotoxemia, and sepsis can cause disseminated intravascular coagulation (DIC). DIC can induce the formation of microvascular thrombi, causing organ failure and death in the absence of the appropriate treatment. To date, there are no drugs to treat DIC, and several reagents have been tested but, their strong doses can lead to severe bleeding, significantly diminishing their benefits. A severe insult like systemic inflammation activates both endothelium and coagulation system. This is characterized by the increment of a widespread of fibrin deposition that is a hallmark in DIC. Our candidate drug, the A2 protein binds to fibrin. In vitro, the A2 protein delayed fibrin formation and blocked platelet adhesion to fibrin(ogen) under high shear stress. Importantly, the A2 protein efficiently reduced the formation of microvascular thrombosis in two different animal species and models for DIC: 1) a murine model for endotoxemia-induced DIC, and 2) a pig model for bacteria-induced DIC. Note that the A2 protein was given to the mice 1.5 hours after the insult with endotoxin, while the A2 protein was administered to the pigs 24 hours after the intravenous inoculation of bacteria. Furthermore, in the murine model the A2 protein prevented organ failure, and markedly improved the survival of the treated mice. Notably, the A2 protein had no effect on platelet aggregation in vitro nor affected the tail bleeding time in mice. The Aims for this STTR are: Aim 1: To determine the pharmacological benefit of the A2 protein on reducing microvascular thrombosis in a porcine model of bacteria-induced sepsis. This aim will demonstrate that the A2 protein is effective in reducing microvascular thrombosis and pro- inflammatory markers using a gram-positive or gram-negative bacteria-induced DIC model. Aim 2: To determine the safety of the A2 protein for hemostasis. This aim will investigate the effect of the A2 protein on hemostasis using large animal models. In Phase II, we will optimize the production of the candidate product, and will perform pharmacokinetic, pharmacological, and toxicological studies. Finally, we will perform time window studies using larger animals.

Project Terms:
activated Protein C; Address; Adhesions; Adverse effects; Affect; Animal Model; Animals; Anti-inflammatory; Antibiotics; Antithrombin III; Autopsy; Bacteria; Binding; Binding Proteins; Bleeding time procedure; Blood Platelets; Burn injury; Centers for Disease Control and Prevention (U.S.); Cessation of life; Clinical; Coagulation Process; Collagen; Critical Illness; Data; Dengue; Deposition; Development; Discipline of obstetrics; Disease; Disseminated Intravascular Coagulation; Dose; drug candidate; Drug Kinetics; Endothelium; Endotoxemia; Endotoxins; Exhibits; falls; Family; Family suidae; Fibrin; Frequencies; Gram-Negative Bacteria; Health; Hemorrhage; Hemostatic function; Heparin; Hour; Human; improved; In Vitro; Inflammation; inflammatory marker; Injury; Intravenous; Lead; Malaria; Malignant Neoplasms; Medical; methicillin resistant Staphylococcus aureus; Modeling; Mono-S; mortality; mouse model; Multiple Organ Failure; Mus; nonhuman primate; novel; novel therapeutic intervention; Organ; Organ failure; Patients; Pharmaceutical Preparations; Pharmacology; Pharmacology and Toxicology; Phase; Platelet aggregation; prevent; Production; Protein C; Proteins; Reagent; Recombinants; Reporting; Safety; Sepsis; shear stress; Small Business Technology Transfer Research; success; Supportive care; Syndrome; System; Tail; Testing; TFPI; Therapeutic; Thrombomodulin; Thrombosis; Thrombus; Time; Toxin; Trauma; Vasculitis; von Willebrand Factor; Work;

Phase II

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
Phase II year
Phase II Amount