SBIR-STTR Award

We have developed and completed a proof-of-principle on a new transgenic mouse model for comprehensive characterization of the antibody repertoire made in response to ovalbumin injection using surface antibody capture and next-generation DNA sequencing technologies. A more commercially valuable proof is now sought using a novel screening method with an emerging drug target for hypercholesterolemia, known as PCSK9.

Thesaurus Terms:
Affinity;Animals;Antibodies;Antibody Affinity;Antibody Repertoire;Antibody-Producing Cells;Antigens;B-Lymphocytes;Base;Binding (Molecular Function);Biological Assay;Biotechnology;Businesses;Cells;Characteristics;Clinical;Clinical Trials;Data;Diagnostic;Dna;Dna Sequence;Drug Targeting;Escherichia Coli;Generations;Genes;Goals;Harvest;Hepatocyte;Human;Hybridomas;Hypercholesterolemia;Il6 Gene;Immune System;Injection Of Therapeutic Agent;Instrument;Label;Legal Patent;Libraries;Light;Low-Density Lipoproteins;Lymphoid Tissue;Marketing;Methods;Monoclonal Antibodies;Mouse Model;Mus;Neutralizing Antibody;Next Generation;Novel;Novel Therapeutic Intervention;Ovalbumin;Pharmacologic Substance;Phase;Property;Proteins;Public Health Relevance;Publishing;Recombinants;Response;Sales;Screening;Services;Speed (Motion);Surface;Surface Plasmon Resonance;System;Technology;Testing;Therapeutic;Transgenic Mice;Transgenic Organisms;Uptake;

We have developed and completed a proof-of-principle on a new transgenic mouse model for comprehensive characterization of the antibody repertoire made in response to PCSK9 injection, and have identified a set of high affinity neutralizing antibodies which will be tested in animals for efficacy and potency.

Public Health Relevance Statement:


Public Health Relevance:
Therapeutic and diagnostic applications of monoclonal antibodies (mAbs) offer a way to target treatments to specific proteins. However, monoclonal antibodies have traditionally been made using hybridoma technologies which only access a small fraction of the immune system. Abeome has developed a novel method of antibody discovery using direct sorting of antigen- specific antibody producing cells. Thus, the immediate goal for this Phase 2 project is to test and improve Abeome's newly discovered antibodies that neutralize the PCSK9/LDLR interaction in animals to determine their efficacy and potency. Our ultimate goal is to develop an immuno- therapeutic for the treatment of hypercholesterolemia and to partner the technology with pharmaceutical/biotechnology companies.

NIH Spending Category:
Atherosclerosis; Biotechnology; Cardiovascular; Digestive Diseases

Project Terms:
Affinity; animal efficacy; Animals; Antibodies; Antibody Repertoire; Antibody-Producing Cells; Antigens; Biological Assay; Biotechnology; Clinical; Diagnostic; Escherichia coli; Evaluation; Goals; Hybridomas; hypercholesterolemia; Immune; Immune system; improved; Injection of therapeutic agent; Methods; Modality; Monoclonal Antibodies; mouse model; neutralizing antibody; novel; novel therapeutic intervention; Pharmacologic Substance; Phase; Proteins; public health relevance; response; Sorting - Cell Movement; Technology; Testing; Therapeutic; Transgenic Mice