Cloning/sequencing/expression of human bsa lipase cdna
Award last edited on: 5/24/02

Sponsored Program
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Principal Investigator
Frederick Hagen

Company Information

Zymogenetics Inc

1201 Eastlake Avenue East
Seattle, WA 98102
   (206) 442-6600
Location: Single
Congr. District: 07
County: King

Phase I

Contract Number: 1R43DK039294-01
Start Date: 00/00/00    Completed: 00/00/00
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Bile salt-activated lipase (BSAL) is found in the milk of a limited number of mammals: humans, gorillas, dogs, and cats. The enzyme plays an important role in the infant diet in the digestion of lipids, causing a striking quantitative difference between breast-fed and formula-fed infants in weight gain. In this regard, it is important to note that BSAL is absent in bovine milk.In Phase I, ZymoGenetics, Inc. plans to clone, sequence, and express the cDNA for human BSAL. This will provide a framework for Phase II research, wherein recombinant human BSAL will be produced in large quantities for supplementation in infant formulas. Expression of the human BSAL cDNA will be achieved by cloning into an expression vector suitable for production at high levels in Saccharomyces cerevisiae. Levels of expression will be ascertained by ELISA and direct measurements of enzymatic activity. The recombinant protein will be purified and characterized for its physical and chemical similarity to the purified human milk enzyme.National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)

Phase II

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
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