Large Scale Purification of Acidic-D-Mannosidase
Profile last edited on: 1/30/14

Total Award Amount
Award Phase
Principal Investigator
Shirish Hirani
Activity Indicator

Company Information

Genzyme Corporation (AKA:Biomatrix Inc~genzyme Biosurgery Corporation)

500 Kendall Square
Cambridge, MA 02142
   (617) 252-7500
Multiple Locations:   
Congressional District:   07
County:   Middlesex

Phase I

Phase I year
Phase I Amount
The specific aim of this Phase I project is to purify to homogeneity acidic a-D-mannosidase from human placentas and bovine kidneys. It is anticipated that by employing a combination of chromatographic techniques on affinity, highperformance anion exchange, and/or high performance gel filtration matrices, homogeneous acidic a-D-mannosidase will be obtained. This will permit the generation of polyclonal and mono-clonal antibodies during Phase II. Furthermore, the purified enzyme and antiserum will provide a means for cloning the gene for acidic a-Dmannosidase. The absence of this enzyme in the lysosomal storage disorder, mannosidosis, which occurs in both man and cattle, makes it an ideal candidate for studying the feasibility of gene replacement therapy.National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMSD)

Phase II

Phase II year
Phase II Amount