Physical Sciences Inc., in collaboration with iRiS Kinetics Inc., will develop a compact and fieldable sensor for the rapid multiplexed detection and presumptive identification of biological threats. The proposed platform is versatile and can simultaneously detect bacterial, viral and toxin threats in diverse environmental samples. The technology will provide highly sensitive and specific multiplexed biothreat detection in less than 1 hr., with greater than 90 percent precision and accuracy. The sensor requires no specialized material or reagent storage and will fill a critical unmet need in biological defense by providing fast and accurate detection with exquisite sensitivity in a compact, ruggedized package.