VitaCyte LLC manufactures highly purified and rigorously characterized tissue dissociating enzymes, which are sold as individual enzyme products or as enzyme formulations for specific cell isolation applications. These enzyme formulations are designed to achieve high viable cell yield, very low contaminant levels, and lot-to-lot consistency. The company's focus is on delivering a custom designed product in applications for isolating human or other mammalian cells from tissue to be used in research or clinical applications. VitaCyte offers purified collagenase and neutral protease products for tissue dissociation on a variety of tissue types. The firmâs CIzyme collagenase enzymes are offered at three different activity levels for applications on different tissue types. This approach reflects the knowledge from improved biochemical assays to understand C. histolyticum collagenase specific collagen degrading activities. Crude collagenases are purified and analyzed using a proprietary purification protocol and improved collagen-degrading assay (CDA) to quantitate each component. Collagenase at different activity levels have the ability to match traditional crude collagenases that work well with certain cell or tissue types using purified components in order to ensure greater reproducibility and benefit from the low endotoxin levels and insoluble components common in most crude collagenases. CIzyme collagenase and neutral protease enzymes are packaged separately to maintain the greatest long-term stability. Rigorous stability data assessments have shown the products to be stable as a lyophilized powder for 2 years when maintained as individual compone
