The primary objective of this proposal is to develop a novel ion source for the efficient on-line coupling of a wide array of chromatographic techniques with mass spectrometry. The use of liquid chromatography separation is a prerequisite for high-throughput mass spectrometry analysis of complex proteomics samples. Commonly used electrospray ionization (ESI), although allowing a direct coupling, imposes significant limitations on the mobile phases and additives used in LC separations. The most widely used separation technique, reversed-phase liquid chromatography, is performed in non-optimal conditions to satisfy operational requirements of the electrospray ion source. The proposed ion source will overcome these limitations and provide efficient analyte ionization from a wide range of liquid sample compositions. Thus, the ion source will allow RP-LC to perform in optimal conditions and also the use of chromatographic techniques not previously amenable by direct ESI-MS analysis.
Public Health Relevance: Proteomics studies biological processes by the systematic analysis of proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new high-throughput technology for the efficient on- line coupling of a wide array of chromatographic separation techniques with mass spectrometry.
Thesaurus Terms: Acetic Acid, Trifluoro-;Affinity;Automation;Biologic Sciences;Biological;Biological Function;Biological Process;Biological Sciences;Body Tissues;Buffers;Cells;Charge;Chromatography;Chromatography / Separation Science;Chromatography, High Performance Liquid;Chromatography, High Pressure Liquid;Chromatography, High Speed Liquid;Chromatography, Molecular Sieve;Complex;Complex Mixtures;Coupled;Coupling;Development;Esi;Electrospray Ionization;Hplc;High Pressure Liquid Chromatography;Hydrophobic Interactions;Ion Exchange;Ions;Life Sciences;Liquid Chromatography;Liquid Substance;Mass Spectrum;Mass Spectrum Analysis;Methods And Techniques;Methods, Other;Molecular Sieve Chromatography;Peptides;Phase;Photometry/Spectrum Analysis, Mass;Play;Procedures;Protein Analysis;Proteins;Proteomics;Reagent;Research;Resolution;Role;Sampling;Size Exclusion Chromatography;Solutions;Source;Spectrometry, Mass;Spectroscopy, Mass;Spectrum Analyses, Mass;Spectrum Analysis, Mass;Speed;Speed (Motion);System;System, Loinc Axis 4;Techniques;Tissues;Trifluoroacetic Acid;Clinical Applicability;Clinical Application;Drug Discovery;Fluid;Gene Product;High Performance Liquid Chromatography;High Throughput Technology;Innovate;Innovation;Innovative;Interest;Ion Source;Ionization;Ionization Method;Ionization Technique;Liquid;Mass Spectrometry;Novel;Social Role;Success
