Current toxicity testing of foodstuffs involves mass spectrometry analysis which requires a >24 h turnaround time in deployed military operations. This length of time is dangerous for personnel. Rapid identification of toxic industrial chemicals (TICs) in food using a sensor that is biologically-based and easy to use eliminates that danger and would provide exactly what is needed for toxicity evaluation of food. In this Phase I, Agave BioSystems proposes to develop a rapid and accurate biosensor using mutant C. elegans to evaluate toxicity in food. This biosensor will use the nematode C. elegans as the model system and measure Green fluorescent protein (GFP) that has been linked to inducible promoters of genes responsive to specific stressors. Extended shelf life will be possible because the dauer stage within the lifecycle of C. elegans leaves the worm in stasis for extended periods of time until growth is resumed with improved conditions. By measuring the GFP response in a mixed population of mutant C. elegans, the outcome should be an accurate and sensitive assessment of chemical toxicity in food.
Keywords: C. Elegans, Food Toxicity, Toxic Industrial Chemicals, Gfp, Gene Mutants, Fluorescence
