SBIR-STTR Award

A Bio-Manufacturing Process For Fabrication Of 3d Scaffolds With Cell Culture Der
Award last edited on: 6/24/10

Sponsored Program
SBIR
Awarding Agency
NIH : NCRR
Total Award Amount
$99,902
Award Phase
1
Solicitation Topic Code
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Principal Investigator
Qing Liu

Company Information

3D Biotek LLC

1 Liene Court Suite 8 Unit 12
Hilsborough, NJ 08844
   (732) 729-6270
   info@3dbiotek.com
   www.3dbiotek.com
Location: Single
Congr. District: 07
County: Somerset

Phase I

Contract Number: 1R43RR028022-01
Start Date: 00/00/00    Completed: 00/00/00
Phase I year
2009
Phase I Amount
$99,902
The objective of this research is to develop novel 3D biodegradable porous polymer scaffolds with biomimetic extracellular matrix (ECM) coating for tissue engineering application. This SBIR Phase I proposal is to demonstrate the scaffold manufacturing feasibility by using a proprietary precision extruding deposition technique to fabricate porous 3D tissue scaffold with controlled porosity and internal architecture, and then coated the scaffold with a cell culture derived extracellular matrix (ECM) by using cell culture technology. We hypotheze that this cell culture derived biomimetic ECM coating, after proper decellularization and treatment, can offer similar biological performance as conventional tissue derived ECM in tissue engineering application. Toward this goal, we would like to demonstrate that an ECM coating can be generated onto the surface of porous tissue scaffolds. To achieve this aim, we first apply an enabling precision extruding deposition technique to freeformly fabricate porous 3D polycaprolactone (PCL) scaffolds with various porous structures. We then apply cell culture method to produce two types of ECM coatings using human dermal fibroblasts and human mesenchymal stem cells (under osteogeneic differentiation). We would like to further demonstrate that the decellularized biomimetic ECM coatings derived from two different cell lines (fibroblast and mesenchymal stem cells under osteogeneic differentiation) will affect osteo-differentiation of re-seeded mesenchymal stem cells. More specifically, we would like to demonstrate that the osteo- differentiated mesenchymal stem cells derived ECM coating could be used as a better ECM substrate for re-seeded mesenchymal stem cell in terms of the cell attachment, proliferation and differentiation down to the osteogeneic lineage. PUBLIC HEALTH RELEVANCE The project is aimed at developing novel 3D biodegradable porous polymer scaffolds with biomimetic extracellular matrix (ECM) coating for tissue engineering application. In this SBIR Phase I work, we will demonstrate the scaffold manufacturing feasibility by using a proprietary fabrication technique to produce porous 3D tissue scaffold with controlled porosity and internal architecture, and then use a cell culture technique to generate an extracellular matrix (ECM) as biomimetic coating. We believe that this novel manufacturing process will produce scaffolds with desired both biophysical properties and biological performance.

Public Health Relevance Statement:
Project Narrative The project is aimed at developing novel 3D biodegradable porous polymer scaffolds with biomimetic extracellular matrix (ECM) coating for tissue engineering application. In this SBIR Phase I work, we will demonstrate the scaffold manufacturing feasibility by using a proprietary fabrication technique to produce porous 3D tissue scaffold with controlled porosity and internal architecture, and then use a cell culture technique to generate an extracellular matrix (ECM) as biomimetic coating. We believe that this novel manufacturing process will produce scaffolds with desired both biophysical properties and biological performance.

NIH Spending Category:
Bioengineering; Biotechnology; Regenerative Medicine; Stem Cell Research; Stem Cell Research - Nonembryonic - Human

Project Terms:
Affect; Architecture; Biochemical; Biological; Biomimetics; Body Tissues; Cell Attachment; Cell Culture Techniques; Cell Line; Cell Lines, Strains; Cell-Extracellular Matrix; Cell-Matrix Adhesions; Cell-Matrix Junction; CellLine; Deposit; Deposition; Dermal; ECM; Electron Microscope; Engineering / Architecture; Extracellular Matrix; Fibroblasts; Goals; Human; Human, General; Light Microscope; Man (Taxonomy); Man, Modern; Mesenchymal Progenitor Cell; Mesenchymal Stem Cells; Methods; Methods and Techniques; Methods, Other; Mimetics, Biological; Performance; Phase; Polymers; Porosity; Property; Property, LOINC Axis 2; Research; SBIR; SBIRS (R43/44); Scanning; Small Business Innovation Research; Small Business Innovation Research Grant; Structure; Surface; Techniques; Technology; Tissue Engineering; Tissues; Work; cultured cell line; engineered tissue; manufacturing process; novel; polycaprolactone; public health relevance; scaffold; scaffolding; tissue scaffold; tissue support frame

Phase II

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
Phase II year
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Phase II Amount
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